Antioxidant proprieties of methanolic and ethanolic. Tocopherol was used as a standard to evaluate the antioxidative activity of samples. The study of phenolic compounds antioxidant activity in. In dpph radical scavenging method the free radicals, 2, 2 diphenyl 1 picrylhydroazyl dpph was used to find antioxidant scavenging activity of various extracts.
Among edible plant materials, remarkable high antioxidant activity and high total phenolic content gae 20 mgg were found in berries, especially aronia and crowberry. Estimation of phytochemical content and antioxidant. Extraction and determination of antioxidant activity of. Pdf evaluation of antioxidant activity of some plant. Screening of in vitro antioxidant activity of methanolic. Antioxidant activity of methanol extracts of different. Proton radical scavenging is an important attribute of antioxidants. A blend of procyanidins with multifaceted therapeutic. This is a good study in which the authors evaluated the antioxidant activity of extracts of root, stem, leaf, flower and fruit of l.
A blend of procyanidins with multifaceted therapeutic applications. The % inhibition of dpph radical by the plant extracts at different concentration increased with increase in concentration of the extract for all samples. Antioxidant activity in a hemoglobininduced linoleic acid peroxidation test the antioxidant activity of extracts was determined by a modified photometry assay25. The samples, with the addition of plant extracts, exhibited higher concentrations of. Among edible plant materials, remarkable high antioxidant activity and high total phenolic content gae 20. Antibacterial and antioxidant activity of traditional. Assessment of antioxidant activity of plant extracts by. The higher the ic 50, the lower is the antioxidant activity of the examined sample. The order of dpph scavenging against cassia fistula extract was found to be in the order of. Screening of plant extracts for antioxidant activity a comparative study on three. The extract can be used effectively for medication purposes. According to recent reports, a highly positive relationship between total phenols and antioxidant activity appears to be the trend in many plant species. The plant leaves exhibited lower antioxidant activity compared to ascorbic acid with 97. Antioxidant compounds like phenolic acids, polyphenols and flavonoids scavenge free radicals such as peroxide.
Particularly plantsourced lowmolecular weight antioxidants such as. A detailed study was performed on the antioxidant activity of the methanol extract. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials. Four different antioxidant assays were used to evaluate aa of the extracts. An antioxidant can be broadly defined as any substance that delays or inhibits oxidative damage to a target molecule 3. Antioxidant activities are known to increase proportionally tothe polyphenol content, mainly due to their redox properties. Pdf antioxidant activity and phenolic content of extracts from. The reactions mechanism involves the electrondonating ability and results in the decolorization of the radical. Ae517 is the absorbance of the plant extract after 1 hour incubation. Journal of medicinal plant research full length research paper antioxidant and anticancer activities of moringa oleifera leaves suphachai charoensin division of biochemistry and nutrition, school of medical sciences, university of phayao, phaholyothin rd. In the previous chapters 3 and 4, it was shown that p. Alcoholic extracts plants soaked in brandy are also extensively used, combining the positive effects of extracted substances with the antiseptic activity of ethanol.
Comparative study of antioxidant properties and total. Antioxidant properties of plant extracts edelweiss publications. The effect of various plant extracts was measured on several important oxidation indicators. Antioxidant activity of plant extracts from colombian flora. Evaluation of antioxidant activity of clitoria ternatea and alternanthera sessilis plant extracts using model system for yeast cells 1balachandar balakrishnan, jayachitra ayyavoo, paramasivam sadayan and arulkumar abimannan 21 1 1department of oceanography and coastal area studies, school of marine sciences. Pdf methods for determining the antioxidant activity. Apple extracts two varieties showed also strong antioxidant activity even though the total phenolic contents were low gae antioxidant activity in a hemoglobininduced linoleic acid peroxidation test the antioxidant activity of extracts was determined by a modified photometry assay25. Evaluation of antioxidant activity of clitoria ternatea. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. Euphorbiaceae is a wide spread family of genus euphorbia, from. The total phenolic contents tpc and the antioxidant activities of water and ethanol extracts of 14 medicinal plants which had been used in iraqi traditional medicine were investigated. Anticancer activity, antioxidant activity, and phenolic and. Antibacterial and antioxidant activity of traditional medicinal plants.
Antioxidant properties of the methanol extracts of the. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. The antioxidant activity of the extracts from mint leaves and carrot was higher at ph 9 than ph 4, while that of drumstick extract remained the same under both ph conditions. Foodbalt 2011 79 antioxidant activities and phenolic composition of extracts from nepeta plant species paulius kraujalis 1, petras rimantas venskutonis 1, ona ragazinskiene2 1kaunas university of technology, department of food technology, radvilenu pl. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate.
Antimicrobial and antioxidant activity of leaf extracts of two indigenous angiosperm species of tripura sunrit basu sarbadhikary1, somnath bhowmik2, badal k datta2 and narayan c mandal1 1mycology and plant pathology laboratory, department of botany, visvabharati, santiniketan 731235, west bengal, india. The antioxidative activity of a total of 92 phenolic extracts from edible and. Owing to the antioxidant and antibacterial activities exhibited by the leaf extracts investigated in this study, this plant could be considered natural antioxidant and antimicrobial agents source that. The antioxidant activities of the methanol extracts from the leaves and stems of celtis africana ulmaceae were assessed in an effort to validate the medicinal potential of the subterranean part of the herb. The study of phenolic compounds antioxidant activity in methanolic and aqueous extracts of several plant species of urmia lake margin parisa faraji, rashid jamei department of biology, faculty of sciences, urmia university, urmia iran available online. Antioxidant activity of antiinflammatory plant extracts request pdf. The antioxidant activity and phenolic contents of the stem as determined by the dpph, proanthocyanidins, total. The study shows the highest antioxidant activity by t. Screening of medicinal plant extracts for antioxidant activity. Antioxidant activities and phenolic contents of the. The extract from carrot was more heatstable than other extracts.
Preliminary phytochemical screening, quantitative analysis. The in vitro antioxidant activity of methanolic stem extract was studied by 22. Antioxidant activity of aqueous, chloroform and methanol extract of the aerial parts of plant oftricholepis glaberrima dc were evaluated for the antioxidant activity by the ftc and tba methods. Specially, methanol and ethanol are high polar among the solvents. The total phenolic content of the extracts was estimated by folin ciocalteau test and total protein. Antimicrobial and antioxidant activity of leaf extracts of. Antioxidant and apoptotic activity of papaya peel extracts. In vitro anticancer, antioxidant and antimicrobial activities of crude methanolic extract of euphorbia cactus ehrenb plant zarraq issa alfaifi abstract. Antioxidant compounds present in plant extracts are therefore multifunctional and their activity and mechanism would largely depend on the composition and conditions of the test system.
Dpph radical scavenging activity was observed in all the extracts, the curcuma extract showed dominant activity followed by coffee bean extract figure 1 among the extracts. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. Screening of plant extracts for antioxidant activity. Medicinal plants, phytochemical constituent, antioxidant activity. The free radical scavenging activity of the plant extracts was measured by dpph 9, with some modifications. The conventional and nonconventional extraction procedures applied prior to. Ethiopia is one of the developing countries which have enormous diversity of plants. Methanolic extract showed higher antioxidant activity than the chloroform and aqueous extract. The main characteristic of an antioxidant is its ability to trap free radicals.
The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Antibacterial and antioxidant activities of 8 aromatic plants from the balkan peninsula were studied. Synergistic antioxidant activity of natural products remedy. Three methods widely employed in the evaluation of antioxidant activity, namely 2,2diphenyl. The lethal dose of the methanolic extract of the plant leaves is more than 2000mgkg. Antioxidant and anticancer activities of moringa oleifera. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. About europe pmc funders joining europe pmc governance roadmap outreach. The results indicate high correlation and regression pvalue antioxidant potentials of the extracts, hence the tut plant could serve as effective free radical inhibitor or scavenger which may be a good candidate for pharmaceutical plant based products. Present study was undertaken to determine phytochemical content and ros scavenging inhibitory activity of 11 medicinal plants from western ghats of. Minimal inhibitory concentration of metanoic extracts ranged from 6.
Therefore, to better understand the global antioxidant capacity of olives and olive oil, methanolic extracts were evaluated by three different assays to cover different mechanisms of the antioxidant defense system. Although earlier reports shows the antimicrobial and antioxidant potential only the. A comparative study on the antioxidant activity of. Antioxidant activity of plant extracts containing phenolic. Evaluation of antioxidant activity of medicinal plant. Plant leaves are rich in flavonols and other pigments. The effect of pp extracts on caspase3 activity magic red caspase 37 detection kit immunochemistry technologies, bloomington, mn and cellular dna fragmentation was determined cell death detection elisa kit, roche diagnostics, indianapolis in according to manufacturers instructions. Evaluation of antioxidant potentials of the methanolic. Specially, the ethanolic extract showed the highest p antioxidant activities % dpph scavenging activity.
Anticancer activity of the plant extracts were also tested on hos and khos. Ba and ca plants containhigher polyphenol levels than the other plants tested table 1. The antioxidant activity was assessed by ferric reducing antioxidant power. Evaluation of antioxidant activity of some plant extracts.
Pdf in vitro antioxidant and antiproliferative activity. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Antioxidant activity increased proportionally to the polyphenol content. Aqueous extracts of 30 plants were investigated for their antioxidant properties using dpph and abts radical scavenging capacity assay, oxygen radical absorbance capacity orac assay, superoxide dismutase sod assay, and ferric reducing antioxidant potential frap assay. Antioxidant capacity determination in plants and plantderived products. Antibacterial and antioxidant efficacy analysis of leaves. Antioxidant activity, total phenolic and total flavonoid. The highest yield of solid residue was obtained using water or methanol as extraction solvents. Reaction mixtures 200 ml containing 10 ml of each extract 10400 mg, 1 mmoll of linoleic acid emulsion, 40 mmoll of phosphate buffer ph 6. Antioxidant activity of plant extracts on the inhibition. Among edible plant materials, remarkable high antioxidant activity and high total. Karthika abstract the aim of present study was to estimate the total phenolic and flavonoid contents in vitro antioxidant potential of methanolic leaf and root extracts of the herb. Extracts were subjected for the evaluation of antioxidant activity by using various in vitro model systems.
The free radical scavenging action of methanol extracts of plant are in the order as desmodium gangeticum amaranthus caudatus solanum nigrum. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of hypochaeris radicata l. In vitro anticancer, antioxidant and antimicrobial. Pdf in the present study, aerial parts of pterospartum tridentatum plants, collected in three locations in portugal, at different vegetative stages. The reducing power activity of methanolic leaf and root extracts of h. Gc and carotene bleaching test bcbt, have been compared with regard to their application in the screening of plant extracts.
The findings indicated promising antioxidant activity of crude extracts of the above plants and needs further exploration for their effective use in both modern and traditional system of medicines. The plant extracts have been shown to have antimicrobial effect 11 and have been used in the ayurveda and siddha medicine 17. There is an increasing trend to replace synthetic antioxidants, which are of safety concern 1,2, with the natural antioxidants available from plant extracts or isolated products of plant origin. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan rahmangul,1,2,3 syedumerjan,1,4 syedfaridullah,3 samiullahsherani,1 andnusratjahan3 1facultyofpharmacy,universityofbalochistan,quetta,pakistan.
These may be medicinal plants, minerals and organic and inorganic matter 1. Antioxidant activity of herbaceous plant extracts protect. If plant extracts contain antioxidant activity, it may clearup free radicals from the body, and boost the immune system to handle the infection or illness effectively. Libyan capparis spinose has good antioxidant activity. Antioxidant activity of the methanol extracts of pulp and seed was also compared to total phenolic content and it was further found that radical scavenging effects of extracts were directly proportional to the phenolic content present in extracts table 1. Antioxidant capacity determination in plants and plantderived. Antioxidant activity of polyphenolic plant extracts mdpi. In the search for sources of natural antioxidants, in recent years some medicinal plants have been extensively studied for their antioxidant activity and radical. Many authors had stressed the need to perform more than one type of antioxidant activity measurement to take into account the various mechanisms of antioxidant. Figure 1 shows the amount of each extract required for 50% inhibition of dpph activity ic 50. Evaluation of antioxidant activity of some plant extracts and their application in biscuits. Antioxidant and in vitro cytotoxic activity of extracts of. Abstract identification and characterization of natural products with antioxidant and antiproliferative activity has received much interest over the past few years.
The yield of extract obtained from 10 g of dry plant material was measured for each extract table 1. Antioxidant activities of aqueous extracts of selected. It is concluded that, the solvent play a vital role in the extraction of the plant constituents. Lightfoot 2 1 department of food science, college of agriculture, university of albasrah, basrah 61004, iraq 2 department of plant, soil and agricultural systems, plant. In vitro antioxidant activity of withania somnifera root. Total phenolic content was also determined by the folin. Comparative analysis of the antioxidant activity of cassia.287 1542 700 324 300 392 913 427 372 251 1156 1078 638 318 194 866 171 465 684 251 754 1145 270 1236 814 333 1501 778 78 519 1454 1342 1428 1375 394 1070 784 231 1464 191